Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD3 , REA1151 , 50 , 130-120-267 , FITC (APC) , Miltenyi Biotec.

Techniques: Imaging

Journal: Cell Reports Medicine

Article Title: SARS-CoV-2 antibodies from children exhibit broad neutralization and belong to adult public clonotypes

doi: 10.1016/j.xcrm.2023.101267

Figure Lengend Snippet:

Article Snippet: FITC Anti-Human CD3 (OKT3) , Tonbo Biosciences , Cat# 35–0037; RRID: AB_2621662.

Techniques: Virus, Recombinant, Staining, Plasmid Preparation, Conjugation Assay, Transfection, Chromatography, Next-Generation Sequencing, Software

Journal: Cell Death Discovery

Article Title: B7-H5 blockade enhances CD8 + T-cell-mediated antitumor immunity in colorectal cancer

doi: 10.1038/s41420-021-00628-4

Figure Lengend Snippet: A Growth curve of MC38 tumors after treatment with monoclonal anti-mouse B7-H5 antibody (B7-H5 mAb) or control IgG. n = 7. The experiments were performed in triplicate. Values are expressed as means (SEMs). ** P < 0.01. B The proportion of CD3 + T and CD8 + T cells in tumor tissues from MC38 tumors after treatment with B7-H5 mAb or control IgG was analyzed by immunofluorescence (original magnification ×200). A representative image was shown. n = 4. The experiments were performed in triplicate. Values are expressed as means (SD). * P < 0.05; ** P < 0.01. C The proportion of CD8 + T cells in the CD45 + T cells of tumor tissues from MC38 tumors after treatment with B7-H5 mAb or control IgG was analyzed by flow cytometry. A representative image was shown. n = 4. The experiments were performed in triplicate.

Article Snippet: Frozen sections from mouse tumor tissues (thickness 5 μm) were blocked with 5% bovine serum albumin for 1 h at 37 °C and stained with anti-mouse CD3 antibody (Proteintech, Wuhan, China) or FITC-conjugated anti-mouse CD8 antibody (Biolegend, San Diego, CA, USA) overnight at 4 °C.

Techniques: Control, Immunofluorescence, Flow Cytometry

Journal: Cell reports

Article Title: The aryl hydrocarbon receptor cell intrinsically promotes resident memory CD8 + T cell differentiation and function

doi: 10.1016/j.celrep.2022.111963

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Article Snippet: Anti-human CD3 – FITC (clone SK7) , TONBO biosciences , Cat# 35-0036-T025; RRID: AB_2621661.

Techniques: Virus, Isolation, Recombinant, SYBR Green Assay, DNA Library Preparation, Reverse Transcription, Staining, Transgenic Assay, Plasmid Preparation, Software